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apoptosis related proteins  (Proteintech)


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    Structured Review

    Proteintech apoptosis related proteins
    Apoptosis Related Proteins, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 4736 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apoptosis+related+proteins/Caspase+3%2Fp17%2Fp19+Antibody/pm41505005-69-49-63
    Average 97 stars, based on 4736 article reviews
    apoptosis related proteins - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: USP39 attenuates the antitumor activity of cisplatin on colon cancer cells dependent on p53.
    Article Snippet: enhances the cisplatin-induced apoptosis in HCT116 cells.. Conversely, overexpression of USP39 attenuates apoptosis in RKO cells.. Furthermore, we demonstrate that USP39 depletion promotes apoptosis induced by cisplatin, which is related with the induction of oxidative stress and DNA damage response.

    Incubation:

    Article Title: Dual-enzymatically cross-linked gelatin hydrogel promotes neural differentiation and neurotrophin secretion of bone marrow-derived mesenchymal stem cells for treatment of moderate traumatic brain injury.
    Article Snippet: Traumatic brain injury (TBI) is one of the most devastating nervous injuries.. Neural tissue engineering based on stem cells and bioactive scaffold is a promising but challenging approach for neural repair.. A cutting-edge system with capability to control the fate of encapsulated stem cells is attractive to enhance neural regeneration after TBI.

    Article Title: X‐Box binding protein 1 downregulates SIRT6 to promote injury in pancreatic ductal epithelial cells
    Article Snippet: Proteins were separated using SDS‐polyacrylamide gel electrophoresis and transferred to PVDF membranes (Roche). .. Next, membranes were blocked with 5% skimmed milk, followed by the incubation with primary antibodies against SIRT6, XBP1, ER stress‐related, apoptosis‐related proteins and HRP‐conjugated secondary antibody (Proteintech group or Abcam). .. Blots were visualized with ECL reagent (Biosharp) and gray values were analyzed using ImageJ software.

    Article Title: Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment
    Article Snippet: Then an equal amount of protein was loaded and separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and transferred to a polyvinylidene difluoride membrane (EMD Millipore, Billerica, MA, USA). .. After that, primary antibodies against neuronal differentiation related proteins (NSE, NeuN, and NFL, Proteintech), neurotrophic factors (BDNF, Proteintech), inflammation-associated protein (IL-6, Proteintech) and apoptosis-related proteins (Bax, Bcl-2, Proteintech) were incubated respectively, followed by horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody. β-actin was used as an internal control. .. The protein analysis was visualized by using Quantity One software (Azure Biosystems C300, Azure c300, USA).

    Article Title: Epigenetic remodeling and apoptotic activation by Chidamide suppress small cell lung cancer in molecularly distinct subtypes.
    Article Snippet: Protein concentration was determined using a BCA kit (Beyotime) measured on a Multiskan GO spectrophotometer (Thermo Fisher). .. Samples (50 μg) were separated by SDS-PAGE (Epizyme Biotech), transferred to PVDF membranes (Millipore), blocked with 5% skim milk, and incubated overnight at 4°C with primary antibodies (1:1000, Cell Signaling Technology) against HDAC1–3, histones (H3, Ac-H3, Ac-H4), DNA damage/repair markers (γ-H2AX, p21, p27), cell cycle regulators (Cyclin E1, CDK2), apoptosis-related proteins (BCL2, BAX, Cytochrome C, PARP, Cleaved-PARP, Caspase-3, Cleaved caspase-3), and GAPDH (1:5000, Proteintech). .. HRP-conjugated secondary antibodies (1:5000, rabbit IgG, Signalway; mouse IgG, Biodragon) were applied for 1 h at RT, and signals were detected by chemiluminescence (MiniChemi imaging system, Sage Creation).

    Control:

    Article Title: Dual-enzymatically cross-linked gelatin hydrogel promotes neural differentiation and neurotrophin secretion of bone marrow-derived mesenchymal stem cells for treatment of moderate traumatic brain injury.
    Article Snippet: Traumatic brain injury (TBI) is one of the most devastating nervous injuries.. Neural tissue engineering based on stem cells and bioactive scaffold is a promising but challenging approach for neural repair.. A cutting-edge system with capability to control the fate of encapsulated stem cells is attractive to enhance neural regeneration after TBI.

    Article Title: Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment
    Article Snippet: Then an equal amount of protein was loaded and separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and transferred to a polyvinylidene difluoride membrane (EMD Millipore, Billerica, MA, USA). .. After that, primary antibodies against neuronal differentiation related proteins (NSE, NeuN, and NFL, Proteintech), neurotrophic factors (BDNF, Proteintech), inflammation-associated protein (IL-6, Proteintech) and apoptosis-related proteins (Bax, Bcl-2, Proteintech) were incubated respectively, followed by horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody. β-actin was used as an internal control. .. The protein analysis was visualized by using Quantity One software (Azure Biosystems C300, Azure c300, USA).

    SDS Page:

    Article Title: Epigenetic remodeling and apoptotic activation by Chidamide suppress small cell lung cancer in molecularly distinct subtypes.
    Article Snippet: Protein concentration was determined using a BCA kit (Beyotime) measured on a Multiskan GO spectrophotometer (Thermo Fisher). .. Samples (50 μg) were separated by SDS-PAGE (Epizyme Biotech), transferred to PVDF membranes (Millipore), blocked with 5% skim milk, and incubated overnight at 4°C with primary antibodies (1:1000, Cell Signaling Technology) against HDAC1–3, histones (H3, Ac-H3, Ac-H4), DNA damage/repair markers (γ-H2AX, p21, p27), cell cycle regulators (Cyclin E1, CDK2), apoptosis-related proteins (BCL2, BAX, Cytochrome C, PARP, Cleaved-PARP, Caspase-3, Cleaved caspase-3), and GAPDH (1:5000, Proteintech). .. HRP-conjugated secondary antibodies (1:5000, rabbit IgG, Signalway; mouse IgG, Biodragon) were applied for 1 h at RT, and signals were detected by chemiluminescence (MiniChemi imaging system, Sage Creation).



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    Proteintech primary antibodies against sirt6, xbp1, er stress-related, apoptosis-related proteins
    X‐Box binding protein 1 <t>(XBP1)</t> negatively regulates SIRT6. (A) Site of XBP1 binding to the SIRT6 promoter according to the JASPAR website. (B) Reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) reveals XBP1 level upon caerulein treatment. (C) Western blot analysis reveals XBP1, GRP78, and CHOP protein levels upon caerulein treatment. (D) RT‐qPCR and (E) western blot analysis reveal XBP1 level after transfection. (F) RT‐qPCR and (G) western blot analysis reveal SIRT6 level in response to XBP1 overexpression or knockdown. (H) Luciferase assay was used to determine SIRT6 promoter activity. (I) Binding of XBP1 to the SIRT6 promoter was determined using chromatin immunoprecipitation (ChIP). (J) The level of CHOP in human pancreatic duct epithelial (HPDE) cells was determined using immunofluorescence assay, magnification 200×. ** p < .01, *** p < .001 versus control or oe‐NC or lgG; ### p < .001 versus sh‐NC.
    Primary Antibodies Against Sirt6, Xbp1, Er Stress Related, Apoptosis Related Proteins, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    X‐Box binding protein 1 (XBP1) negatively regulates SIRT6. (A) Site of XBP1 binding to the SIRT6 promoter according to the JASPAR website. (B) Reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) reveals XBP1 level upon caerulein treatment. (C) Western blot analysis reveals XBP1, GRP78, and CHOP protein levels upon caerulein treatment. (D) RT‐qPCR and (E) western blot analysis reveal XBP1 level after transfection. (F) RT‐qPCR and (G) western blot analysis reveal SIRT6 level in response to XBP1 overexpression or knockdown. (H) Luciferase assay was used to determine SIRT6 promoter activity. (I) Binding of XBP1 to the SIRT6 promoter was determined using chromatin immunoprecipitation (ChIP). (J) The level of CHOP in human pancreatic duct epithelial (HPDE) cells was determined using immunofluorescence assay, magnification 200×. ** p < .01, *** p < .001 versus control or oe‐NC or lgG; ### p < .001 versus sh‐NC.

    Journal: Immunity, Inflammation and Disease

    Article Title: X‐Box binding protein 1 downregulates SIRT6 to promote injury in pancreatic ductal epithelial cells

    doi: 10.1002/iid3.1301

    Figure Lengend Snippet: X‐Box binding protein 1 (XBP1) negatively regulates SIRT6. (A) Site of XBP1 binding to the SIRT6 promoter according to the JASPAR website. (B) Reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) reveals XBP1 level upon caerulein treatment. (C) Western blot analysis reveals XBP1, GRP78, and CHOP protein levels upon caerulein treatment. (D) RT‐qPCR and (E) western blot analysis reveal XBP1 level after transfection. (F) RT‐qPCR and (G) western blot analysis reveal SIRT6 level in response to XBP1 overexpression or knockdown. (H) Luciferase assay was used to determine SIRT6 promoter activity. (I) Binding of XBP1 to the SIRT6 promoter was determined using chromatin immunoprecipitation (ChIP). (J) The level of CHOP in human pancreatic duct epithelial (HPDE) cells was determined using immunofluorescence assay, magnification 200×. ** p < .01, *** p < .001 versus control or oe‐NC or lgG; ### p < .001 versus sh‐NC.

    Article Snippet: Next, membranes were blocked with 5% skimmed milk, followed by the incubation with primary antibodies against SIRT6, XBP1, ER stress‐related, apoptosis‐related proteins and HRP‐conjugated secondary antibody (Proteintech group or Abcam).

    Techniques: Binding Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Transfection, Over Expression, Knockdown, Luciferase, Activity Assay, Chromatin Immunoprecipitation, Immunofluorescence, Control

    X‐Box binding protein 1 (XBP1) curbs the impacts of SIRT6 on the inflammation and oxidative stress. Cells were subjected to simultaneous transfection with SIRT6 and XBP1 overexpression plasmids. (A) the levels of inflammatory factors in the cell supernatant were measured upon caerulein treatment. (B) The dichlorodihydrofluorescein diacetate (DCFH‐DA) probe was used to quantify reactive oxygen species (ROS) levels in human pancreatic duct epithelial (HPDE) cells, magnification 200×. (C) Oxidative stress was assessed by measuring malondialdehyde (MDA) level, superoxide dismutase (SOD), and catalase (CAT) activities. *** p < .001 versus control; ### p < .001 versus caerulein; @ p < .05, p @ @ < .01, @@@ p < .001 versus caerulein + oe‐SIRT6 + oe‐NC.

    Journal: Immunity, Inflammation and Disease

    Article Title: X‐Box binding protein 1 downregulates SIRT6 to promote injury in pancreatic ductal epithelial cells

    doi: 10.1002/iid3.1301

    Figure Lengend Snippet: X‐Box binding protein 1 (XBP1) curbs the impacts of SIRT6 on the inflammation and oxidative stress. Cells were subjected to simultaneous transfection with SIRT6 and XBP1 overexpression plasmids. (A) the levels of inflammatory factors in the cell supernatant were measured upon caerulein treatment. (B) The dichlorodihydrofluorescein diacetate (DCFH‐DA) probe was used to quantify reactive oxygen species (ROS) levels in human pancreatic duct epithelial (HPDE) cells, magnification 200×. (C) Oxidative stress was assessed by measuring malondialdehyde (MDA) level, superoxide dismutase (SOD), and catalase (CAT) activities. *** p < .001 versus control; ### p < .001 versus caerulein; @ p < .05, p @ @ < .01, @@@ p < .001 versus caerulein + oe‐SIRT6 + oe‐NC.

    Article Snippet: Next, membranes were blocked with 5% skimmed milk, followed by the incubation with primary antibodies against SIRT6, XBP1, ER stress‐related, apoptosis‐related proteins and HRP‐conjugated secondary antibody (Proteintech group or Abcam).

    Techniques: Binding Assay, Transfection, Over Expression, Control

    X‐Box binding protein 1 (XBP1) curbs the impacts of SIRT6 on the apoptosis. Cells were subjected to simultaneous transfection with SIRT6 and XBP1 overexpression plasmids. (A) Flow cytometry was used to detect cell apoptosis. (B) Caspase 3 activity was also used to assess apoptosis. (C) Western blot analysis reveals the protein levels of Bcl‐2 and Bax. *** p < .001 versus control; ### p < .001 versus caerulein; @ p < .05, p @ @ < .01, p @ @ @ < .001 versus caerulein + oe‐SIRT6 + oe‐NC.

    Journal: Immunity, Inflammation and Disease

    Article Title: X‐Box binding protein 1 downregulates SIRT6 to promote injury in pancreatic ductal epithelial cells

    doi: 10.1002/iid3.1301

    Figure Lengend Snippet: X‐Box binding protein 1 (XBP1) curbs the impacts of SIRT6 on the apoptosis. Cells were subjected to simultaneous transfection with SIRT6 and XBP1 overexpression plasmids. (A) Flow cytometry was used to detect cell apoptosis. (B) Caspase 3 activity was also used to assess apoptosis. (C) Western blot analysis reveals the protein levels of Bcl‐2 and Bax. *** p < .001 versus control; ### p < .001 versus caerulein; @ p < .05, p @ @ < .01, p @ @ @ < .001 versus caerulein + oe‐SIRT6 + oe‐NC.

    Article Snippet: Next, membranes were blocked with 5% skimmed milk, followed by the incubation with primary antibodies against SIRT6, XBP1, ER stress‐related, apoptosis‐related proteins and HRP‐conjugated secondary antibody (Proteintech group or Abcam).

    Techniques: Binding Assay, Transfection, Over Expression, Flow Cytometry, Activity Assay, Western Blot, Control